南京农大生命科学学院导师介绍：杨清

　　五、研究概述
　　1984-2000年，在法国和加拿大留学、工作，从事十字花科植物雄性生殖、单倍体、生物技术、分子生物学等研究。
　　2000年至今，在南京农业大学工作，从事植物功能基因组学、基因工程、马玲薯生物技术和分子改良研究。先后承担国家基金委、教育部、科技部、外专局和省科技厅课题十多项。通过以上项目以培养博士研究生17名、硕士研究生39名。
　　主要成果：提出花椰菜离体雄性生殖的模型与机理; 建立了花椰菜小孢子单倍体再生体系，首次成功获得单倍体植株; 建立了高效花药悬浮培养体系; 建立了单倍体快速检测鉴定方法; 创立了花蕾培养诱导单倍体新方法; 建立了落叶松成熟材料原生质体再生体系和再生潜力细胞筛选的数学模型; 利用不同倍体材料分析并提出马铃薯自交不亲和性的新证据，进一步完善了自交不亲和性的分子机理; 在马铃薯功能基因研究方面, 克隆了与块茎发育光调控相关基因7个, 花色苷生物合成基因6个调控基因1个, 抗虫基因2个; 在茄子抗黄萎病研究方面, 克隆了与防卫反应相关基因9个; 在水稻耐盐性遗传方面，克隆了与耐盐相关的新基因2个; 在转基因研究方面, 先后获得了MSI-99转基因马铃薯, 转中长链羟基脂肪酸聚酯合成基因烟草, OsGA20ox2 RNAi半矮干水稻, 转花青苷生物合成关键酶基因3GT马铃薯, 转StoVe1马铃薯, 转PGIP马铃薯, antisense-PHYB马铃薯, antisense-StCOL马铃薯等; 在洋葱CMS不育性研究方面,揭示了CMS不育机理; 在其它方面, 研究了慈姑细胞骨架形成机制、大白菜对软腐病菌的防卫机制、野生茄子抗黄萎病的分子机制以及马铃薯花色苷抗氧化机制。 发表学术论文100余篇。

　　六、近期论文
　　- Hong YB, Liu SP, Zhu YP, Xie C, Jue DW, Chen M, Kaleri HA and Yang Q (2012) Expression of the MSI-99m Gene in Transgenic Potato Plants Confers Resistance to Phytophthora infestans and Ralstonia solanacearum． Plant Mol Biol Rep, DOI 10．1007/s11105-012-0503-x
　　- Liu SP, Zhu YP, Xie C, Jue DW, Hong YB, Chen M, Hubdar AK, Yang Q(2012)Transgenic Potato Plants Expressing StoVe1 Exhibit Enhanced Resistance to Verticillium dahliae． Plant Mol Biol Rep, 30:1032-1039
　　- Wei Q, Feng ZH, Wang QY, Wang B, Zhang YF, Yang Q (2012) Increased accumulation of anthocyanins in transgenic potato tubers by over-expression of 3GT gene． Plant Biotechnol Rep，6: 69–75
　　- Wang QY, Chen Q, He ML, Mir PY, Su JY and Yang Q (2011): Inhibitory effect of antioxidant extracts from various potatoes on the proliferation of human colon and liver cancer cells, Nutr Cancer． 63(7):1044-1052
　　- Xie YJ, Cui WT, Yuan XX, Shen WB, Yang Q (2011) Haem Oxygenase-1 is Associated with Wheat Salinity Acclimation by Modulating Reactive Oxygen Species Homeostasis． Journal of Integrative Plant Biology, 53( 8): 653-670
　　- Xie YJ, Xu S, Han B, Wu MZ, Yuan XX, Han Y, Gu Q, Xu DK, Yang Q and Shen WB (2011) Evidence of Arabidopsis salt acclimation induced by up-regulation of HY1 and the regulatory role of RbohD-derived reactive oxygen． species synthesis． Plant Journal, 66(2)：280-292
　　- Li C, Huang B, Yan XH, Wang LJ, Yang Q, and Wei WH, Cloning, identification and characterization of a repetitive sequence flanking telomere and homologous to canrep in Brassica napus． Botanical Studies，2010, 51: 421-430
　　- Guo JL, Yu CL, Fan CY, Lu QN, Yin JM, Zhang YF, Yang Q(2010)Cloning and characterization of a potato TFL1 gene involved in tuberization regulation． Plant cell, tissue and organ Culturue, 103:103-109
　　- Zhang F, Wang Z, Huang QS, Huang LP, Yang Q (2010) Cloning and Characterization of a PGIP-encoding Gene from Solanum torvum． Agriculrural Sciences in China, 9(6)：921-927
　　- Wang Z, GuoJL, ZhangF, Huang QS, HuangLP, and YangQ(2010) Differential Expression Analysis by cDNA-AFLP of Solanum torvum upon Verticillium dahliae Infection． Russ． J． of Plant Physiology, 57(5)：676-684
　　- Wang Z, Chen CQ, Yuan XX, Xu S, Yang Q, Shen WB (2009) Mitigative effect of exogenous carbon monoxide on cell death in rice suspension-cultured celld under salt stress． Plant Physiology Communications, 45(7):643-646
　　- Zeng BY, Wang Z, Zhang YF, Yang Q, Lu W． (2009) Cryopreservation of rice (Oryza sativa L．) embryonic cell suspensions by encapsulaition-dehydration． Plant Physiology Communications, 45(6):603-606
　　- Hong He, Rachid Serraj, Qing Yang(2009)Changes in OsXTH gene expression, ABA content, and peduncle elongation in rice subjected to drought at the reproductive stage． Acta Physiol Plant 31(4):749–756
　　- Liu SQ, Xu L, Jia ZQ, Xu Y, Yang Q, Fei ZJ, Chen HM, Huang SW (2008) Genetic association of ETHYLENE-INSENSITIVE3-like sequence with the sex-determining M locus in cucumber (Cucumis sativus L．)． Theor Appl Genet． 117:927-933
　　- Guo JL, Yang Q． (2008) Molecular cloning and expression analysis of a LFY homologous gene from poato． Plant Mol Biol Rep 26:324-334
　　- Liang F, Shen LZ, Chen M and Yang Q (2008) Formation of intercellular gas space in the diaphragm during the development of aerenchyma in the leaf petiole of Sagittaria trifolia． Aquatic Botany, 88(3):185-195
　　- Liang F, Wang Z, Guo JL, Shen LZ and Yang Q (2008) Dynamic changes of mineral element in the cell wall of growth cells detected by CSEM-EDX． Pregress in Biochemistry and biophysics, 35(2):1-11
　　- ZOU HW, WU ZY, ZHANG XH, WANG YQ, YANG Q, CAO MQ, and HUANG CL (2008) Cloning and Characterization of Maize ZmASK1, a Homologue to Shaggy/GSK-3-like Gene, Involved in Plant Responses to Abiotic Stresses． ACTA AGRONOMICA SINICA, 34(2): 184−191
　　- Zhang JJ, Hao GP, Wu ZY, Zhang XH, Wang YQ, Yang Q, Cao MQ, Liu SG and Huang CL (2008) Nucleotide variation in ATHK1 region of Arabidopsis thaliana and its association study with drought tolerance． African Journal of Biotechnology, 7 (3): 224-233
　　- Xing YJ, Yang Q, Ji Q, Luo YM, Zhang YF, Gu K and Wang DZ (2007)Optimization of Agrobacterium-mediated transformation parameters for sweet potato embryogenic callus using-glucuronidase (GUS) as a reporter． African Journal of Biotechnology , 6 (22): 2578-2584
　　- Guo JL，Yang Q，Liang F, Xing YJ and Wang Z (2007) Molecular Cloning and Expression Analysis of a Novel CONSTANS-like From Potato． Biochemistry, 72(11)：1241-1246
　　- GUO Q, XIANG AL, YANG Q and YANG ZM(2007)Bioinformatic identification of microRNAs and their target genes from Solanum tuberosum expressed sequence tags． Chinese Science Bulletin，52(17)：2380-2389
　　- Zhang SH, Yang Q and Ma RC (2007) Erwinia carotovora ssp． carotovora Infection Induced "Defense Lignin" Accumulation and Lignin Biosynthetic Gene Expression in Chinese Cabbage (Brassica rapa L． ssp． pekinensis)． Journal of Integrative Plant Biology, 49 (7): 993-1002
　　- Qiao F, Yang Q, Wang CL, Fan YL, Wu XF and Zhao KJ (2007) Modification of plant height via RNAi suppression of OsGA20ox2 gene in rice． Euphytica, 158 (1-2): 35-45
　　- Jiang MH, Huang XQ, Li L, Li Y and Yang Q (2007) Effects of genotype and hormones on the regeneration of transgenic potato plant． Seeds, 26(8): 1-4
　　- Zhang SH, Yang Q and Ma RC (2006) Isolation, characterization and expression analysis of BrMyb from Erwinia carotovora subsp． Carotovora diseased Chinese cabbage． African Journal of Biotechnology, 6 (5): 516-522
　　- Zhang Y, Guo JL, Lu QN and Yang Q (2006) Cloning and expression analysis of PHYA cDNA from wild potato (Solanum pinnatisectum)． Plant Physiology Communications, 42(6): 1081-1086
　　- Xiao X．, Zhang XH, Yang Q, Zou HW, Wu ZY, Yu R, Cao MQ and Huang CL (2006) Subcellular Localization of ZmSPK1, A Protein Kinase from Maize, in Transgenic Arabidopsis． Acta Agriculturae Boreali-Sinica, 21(3): 1-4
　　- Li YY, Wang Z, Liang F, Shen LZ, Yan JY, and Yang Q (2006) RAPD analysis of the genomic DNA of CMS line 70 and its maintainer line 71 in onion． Biotechnology Bulletin, 6: 100-102
　　- Lu QN，Yang Q and Zou HW(2006)Effects of cerium on the accumulation of anthocyanins and expression of anthocyanin biosynthetic genes in in-vitro potato tissue ． Journal of rare earths, 24(4):479-484
　　- Lu QL and Yang Q (2006) Cloning and expression of anthociaynin biosynthetic genes in wild potato． African Journal of Biotechnology , 5 (10): 811-818
　　- Zou HW, Wu ZY, Xiao X, Zhang XH, Cao MQ, Jia WS, Huang CL and Yang Q (2006) Gene expression analyses of ZmPti1, encoding a maize Pti-like kinase, suggest a role in stress signaling． Plant Science 171: 99-105
　　- Zou HW, Yang Q, Zhang XH, Zhao JR, Wu ZY, Wang FG and Huang CL (2006) Cloning and Characterization of Maize ZmSPK1, a Homologue to Nonfermenting1-Related Protein Kinase2． African Jounal of Biotechnology 5 (6): 490-496
　　- Shi RJ, Yin Y, Wang Z, Chen M and Yang Q (2006) Cloning and analysis of full-length cDNA of StoVe1 gene from Solanum torvum． Plant Physiology Communication 44(4):638-642 (in Chinese)
　　- Xu LL, Lu D, Lu W, Zhang RX and Yang Q (2006) Establishment of suspension cell line of rice (Oryza sativa L．) and effects of different media on biomass． Plant Physiology Communication 44(4):612-616 (in Chinese)
　　- Li Y Y, Yang Q and Yan J Y and Chen M (2006) Cytoplasmic Male-Sterility of Onion (Allium cepa L．) 63A Is Associated with Premature Degeneration of the Tapetum． Acta Agronomica Sinica 32(3):369-372
　　- Zhang y, Yang Q and Chen M (2006) In-vitro production of microtubers from wild potato． Acta Horticulturae Sinica 33(2):369
　　- Wang YH, Wu ZY, Zhang XH, Huang CL and Yang Q (2006) Stable transfortion of phaC2 gene in tobacco chloroplast genome and maternal inheritance of transgenes． Journal of Agricultural Biotechnology 14(2):213-218(in Chinese)
　　- Shi RJ, Zhao ML and Yang Q (2006) Cloning and analysis of glutathione reductase gene from Leymus multicaulis．Journal of Northwest Sci-tech University of Agriculture and Forestry 34(2):61-67(in Chinese)
　　- Qiao F, Fan YL, Yang Q and Zhao KJ (2006) RNAi molecular mechanism and its application in plant functional genomics research． Biotechnology belletin 4: 20-24
　　- Xiao X , Zhang X , Yang Q , Zou HW , Wu ZY , Yu R , Cao MQ and Huang CL (2006) Subcellular Localization of ZmSPK1 , A Protein Kinase from Maize , in Transgenic Arabidopsis． Acta Agriculturae Boreali-Sinica 21(3):1-4
　　- Wang YH, Wu ZY, Zhang XH, Chen GQ, Wu Q, Huang CL and Yang Q (2005) Synthesis of medium-chain-length-polyhydroxyalkanoates in tobacco via chloroplast genetic engineering． Chinese Science Belletin 50 (11): 1113-1120
　　- Shi RJ, Zhao ML and Yang Q (2005) Cloning and Analysis of cDNA segment of glutathione reductase in Leymus multicaulis． Acta Bot． Boreal．-Occcident． Sin． 25(5): 881-886 (in Chinese)
　　- Zhao CL, Guo WM, Yang Q and Chen JY (2005) Cloning of full-length F3’H of Nanjing hongxu (Prunus Mume) by gDNA-based TAIL-PCR． Acta Bot． Boreal．-Occcident． Sin．25(12): 2378-2385

　　七、科研合作
　　1． 加拿大农业与农业食品部马铃薯研究中心
　　2． 中国农业科学院蔬菜花卉研究所

　　八、讲授课程
　　1． 现代分子生物学(硕士/博士, 获校级(2011)和省级(2012)优秀研究生课程)
　　2． 分子生物学研究方法(硕士/博士,2005年校级研究生重点建设课程)
　　3． 分子细胞生物学(硕士,博士)
　　4． 分子生物学研究进展(博士)
　　5． 细胞工程(本科)

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